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donkey anti goat igg h l  (Bio-Rad)


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    Structured Review

    Bio-Rad donkey anti goat igg h l
    Donkey Anti Goat Igg H L, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rat+igg/Donkey+anti+Goat+IgG+(H%2FL)+(Rat%2FMouse+Adsorbed)/bio_rxiv__64898__2026__04__27__720799-52-17-15
    Average 93 stars, based on 31 article reviews
    donkey anti goat igg h l - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: A novel ceRNA regulatory network involving the long noncoding NEAT1, miRNA-466f-3p and its mRNA target in osteoblast autophagy and osteoporosis.
    Article Snippet: Osteoporosis (OP) is a systemic metabolic disorder characterized by a reduction in bone tissue volume.. LncRNAs have been reported to act as regulators of several human diseases.. Specifically, lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) is involved in proliferation, differentiation and apoptosis in osteoclasts and bone marrow mesenchymal stem cells and regulates the occurrence and development of OP.

    Article Title: HpBoRB, a helminth-derived CCP domain protein which binds RELMβ.
    Article Snippet: Binding was detected by probing for tags found on the respective proteins, anti-FLAG (Biolegend; 1:2,500 in 2 % BSA PBST), and anti-rat CD4d3+4 antibody (Bio-rad; 1:1,000 in 2 % BSA PBST) for 1 hr at RT. .. After washing, goat anti-rat IgG, HRP conjugate (Abcam; 1:5,000 in 2 % BSA PBST) and goat anti-mouse IgG, HRP conjugate (Bio-rad; 1:2,500 in 2 % BSA PBST) was added and incubated for 1 hr at RT. ..

    other:

    Article Title: "Exploring the Main Source of Coproporphyrins: Observations on Transport in Red Blood Cells"
    Article Snippet: 841 Primary antibody (Ab) Dilution primary Ab Secondary Ab Dilution secondary Ab BCRP (BXP-21, GTX23380†) 1:250 goat anti-mouse IgG (GTX23380‡) 1:2000 0.5% milk-TBST ABCB6 (SAB1300078*) 1:500 goat anti-rabbit IgG (172-1019‡) 1:2000 MRP4 (M4I-80 #) 1:1000 goat anti-rat IgG (sc-2032■) 1:2000 Na+/K+-ATPase (ab76020▲) 1:2000 goat anti-rabbit IgG (172-1019‡) 1:2000 † Genetex, Irvine, USA, *Sigma, Buchs, CH, ‡ BioRad Laboratories, Cressier, Switzerland; ▲ 842 abcam, Cambridge, UK, ■ Santa Cruz, Dallas, USA, # Novus Biologicals, Littleton, USA, bovine 843 serum albumin (BSA).

    Article Title: Early intervention anti-Aβ immunotherapy attenuates microglial activation without inducing exhaustion at residual plaques
    Article Snippet: Goat anti-Rat IgG (H/L): HRP , (1:1000) , 5204 − 2504 , Biorad.

    Chromatin Immunoprecipitation:

    Article Title: Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-B55α (PPP2R2A (2G9), Cell Signaling Technologies, Mouse mAb #5689, 1:2000) Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) 3 nature portfolio | reporting sum m ary April 2023 Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PP2Ac (Anti-PP2A Antibody, C subunit, clone 1D6, Millipore Sigma, Cat#05-421, 1:2000) YFP (in-house generated, 1:5000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-PP2Ac Methyl (Leu309) (Clone 2A10, Biolegend, Cat # 828801, Lot: B349332, 1:1000) Anti-FAM122A (Clone 3E9, ThermoFisher Scientific, Cat# MA5-24510, 1:1000) Anti-ARPP19 (Proteintech, Cat#11678-1-AP, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/ppp2r2a-2g9-mouse-mab/5689?_requestid=7384374 PMID: 36781846, PMID: 35695070) Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Article Title: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-StrepII (Genscript, Cat # A01732, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Flow Cytometry:

    Article Title: Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-B55α (PPP2R2A (2G9), Cell Signaling Technologies, Mouse mAb #5689, 1:2000) Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) 3 nature portfolio | reporting sum m ary April 2023 Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PP2Ac (Anti-PP2A Antibody, C subunit, clone 1D6, Millipore Sigma, Cat#05-421, 1:2000) YFP (in-house generated, 1:5000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-PP2Ac Methyl (Leu309) (Clone 2A10, Biolegend, Cat # 828801, Lot: B349332, 1:1000) Anti-FAM122A (Clone 3E9, ThermoFisher Scientific, Cat# MA5-24510, 1:1000) Anti-ARPP19 (Proteintech, Cat#11678-1-AP, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/ppp2r2a-2g9-mouse-mab/5689?_requestid=7384374 PMID: 36781846, PMID: 35695070) Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Article Title: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-StrepII (Genscript, Cat # A01732, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Magnetic Resonance Imaging:

    Article Title: Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-B55α (PPP2R2A (2G9), Cell Signaling Technologies, Mouse mAb #5689, 1:2000) Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) 3 nature portfolio | reporting sum m ary April 2023 Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PP2Ac (Anti-PP2A Antibody, C subunit, clone 1D6, Millipore Sigma, Cat#05-421, 1:2000) YFP (in-house generated, 1:5000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-PP2Ac Methyl (Leu309) (Clone 2A10, Biolegend, Cat # 828801, Lot: B349332, 1:1000) Anti-FAM122A (Clone 3E9, ThermoFisher Scientific, Cat# MA5-24510, 1:1000) Anti-ARPP19 (Proteintech, Cat#11678-1-AP, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/ppp2r2a-2g9-mouse-mab/5689?_requestid=7384374 PMID: 36781846, PMID: 35695070) Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Article Title: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-StrepII (Genscript, Cat # A01732, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Generated:

    Article Title: Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-B55α (PPP2R2A (2G9), Cell Signaling Technologies, Mouse mAb #5689, 1:2000) Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) 3 nature portfolio | reporting sum m ary April 2023 Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PP2Ac (Anti-PP2A Antibody, C subunit, clone 1D6, Millipore Sigma, Cat#05-421, 1:2000) YFP (in-house generated, 1:5000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-PP2Ac Methyl (Leu309) (Clone 2A10, Biolegend, Cat # 828801, Lot: B349332, 1:1000) Anti-FAM122A (Clone 3E9, ThermoFisher Scientific, Cat# MA5-24510, 1:1000) Anti-ARPP19 (Proteintech, Cat#11678-1-AP, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/ppp2r2a-2g9-mouse-mab/5689?_requestid=7384374 PMID: 36781846, PMID: 35695070) Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Biomarker Discovery:

    Article Title: Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-B55α (PPP2R2A (2G9), Cell Signaling Technologies, Mouse mAb #5689, 1:2000) Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) 3 nature portfolio | reporting sum m ary April 2023 Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PP2Ac (Anti-PP2A Antibody, C subunit, clone 1D6, Millipore Sigma, Cat#05-421, 1:2000) YFP (in-house generated, 1:5000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-PP2Ac Methyl (Leu309) (Clone 2A10, Biolegend, Cat # 828801, Lot: B349332, 1:1000) Anti-FAM122A (Clone 3E9, ThermoFisher Scientific, Cat# MA5-24510, 1:1000) Anti-ARPP19 (Proteintech, Cat#11678-1-AP, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/ppp2r2a-2g9-mouse-mab/5689?_requestid=7384374 PMID: 36781846, PMID: 35695070) Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Article Title: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging 3 nature portfolio | reporting sum m ary April 2023 Antibodies Antibodies used Anti-B55α (PP2A B Subunit (100C1) Rabbit mAb, Cell Signaling Technologies, Cat # 2290S, 1:1000) Anti-PP2Ac alpha/beta (Clone 11H12, Mouse mAb, Millipore, Cat# MABE1783, Lot# Q3046542, 1:1000) Anti-PPP2R1A (Clone 6G3, Biolegend, Cat# 824901, 1:1000) Anti-StrepII (Genscript, Cat # A01732, 1:1000) Goat anti-Rat IgG, DyLight 800 (ThermoFisher Scientific, Cat# SA5-10024, 1:3000) Goat anti-Rabbi IgG, StarBright Blue 520 (Bio-Rad, Cat# 12005869, 1:3000) Goat anti-Mouse IgG, StarBright Blue 700 (Bio-Rad, Cat# 12004158, 1:3000) Validation All antibodies used in this study are commercially available and have been validated by the manufacturer and previous publications Anti-B55α (https://www.cellsignal.com/products/primary-antibodies/pp2a-b-subunit-100c1-rabbit-mab/2290. .. PMID: 34911954, 34794320) Anti-PP2Ac (https://www.emdmillipore.com/US/en/product/Anti-PP2AC-alpha-beta-Antibody-clone-11H12,MM_NFMABE1783-25UL.)

    Concentration Assay:

    Article Title: Early intervention anti-Aβ immunotherapy attenuates microglial activation without inducing exhaustion at residual plaques.
    Article Snippet: Membranes were developed using Pierce ECL Western Blotting-Substrate (Thermo Scientific, 32106) and signals visualised using autoradiographic development using Fujifilm Medical X-ray Film Super RX-N (Fujifilm, 47410) or using the Amersham ImageQuant 800. .. Primary antibody Concentration Catalog number Company Rat anti-Aβ (2D8) 1:50 from hybridoma supernatant n/a n/a Rat anti-TREM2 (5F4) 1 μg/mL (1:1000) n/a In-house, see [27] Rabbit-anti-APP (Y188) 0.384 μg/mL (1:1000) ab32136 Abcam Secondary antibody Concentration Catalog number Company Goat anti-Rat IgG (H/L): HRP (1:1000) 5204 − 2504 Biorad Goat anti-Rabbit IgG (H/L): HRP (1:1000) 5196 − 2504 Biorad Magnetic-activated microglia sorting (MACS) from mouse brain Prior to microglia isolation, meninges were removed by gently rolling brains on a clean piece of Whatman paper. ..

    Magnetic Cell Separation:

    Article Title: Early intervention anti-Aβ immunotherapy attenuates microglial activation without inducing exhaustion at residual plaques.
    Article Snippet: Membranes were developed using Pierce ECL Western Blotting-Substrate (Thermo Scientific, 32106) and signals visualised using autoradiographic development using Fujifilm Medical X-ray Film Super RX-N (Fujifilm, 47410) or using the Amersham ImageQuant 800. .. Primary antibody Concentration Catalog number Company Rat anti-Aβ (2D8) 1:50 from hybridoma supernatant n/a n/a Rat anti-TREM2 (5F4) 1 μg/mL (1:1000) n/a In-house, see [27] Rabbit-anti-APP (Y188) 0.384 μg/mL (1:1000) ab32136 Abcam Secondary antibody Concentration Catalog number Company Goat anti-Rat IgG (H/L): HRP (1:1000) 5204 − 2504 Biorad Goat anti-Rabbit IgG (H/L): HRP (1:1000) 5196 − 2504 Biorad Magnetic-activated microglia sorting (MACS) from mouse brain Prior to microglia isolation, meninges were removed by gently rolling brains on a clean piece of Whatman paper. ..

    Isolation:

    Article Title: Early intervention anti-Aβ immunotherapy attenuates microglial activation without inducing exhaustion at residual plaques.
    Article Snippet: Membranes were developed using Pierce ECL Western Blotting-Substrate (Thermo Scientific, 32106) and signals visualised using autoradiographic development using Fujifilm Medical X-ray Film Super RX-N (Fujifilm, 47410) or using the Amersham ImageQuant 800. .. Primary antibody Concentration Catalog number Company Rat anti-Aβ (2D8) 1:50 from hybridoma supernatant n/a n/a Rat anti-TREM2 (5F4) 1 μg/mL (1:1000) n/a In-house, see [27] Rabbit-anti-APP (Y188) 0.384 μg/mL (1:1000) ab32136 Abcam Secondary antibody Concentration Catalog number Company Goat anti-Rat IgG (H/L): HRP (1:1000) 5204 − 2504 Biorad Goat anti-Rabbit IgG (H/L): HRP (1:1000) 5196 − 2504 Biorad Magnetic-activated microglia sorting (MACS) from mouse brain Prior to microglia isolation, meninges were removed by gently rolling brains on a clean piece of Whatman paper. ..



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    Bio-Rad donkey anti goat igg h l
    Donkey Anti Goat Igg H L, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
    donkey anti goat igg h l - by Bioz Stars, 2026-09
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    Vector Laboratories biotinylated goat anti rat igg
    Biotinylated Goat Anti Rat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 1 article reviews
    biotinylated goat anti rat igg - by Bioz Stars, 2026-09
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    Vector Laboratories secondary antibody solution
    Secondary Antibody Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rat+igg/Biotinylated+Goat+Anti-Rat+IgG+Antibody/pm42034823-319-15-27
    Average 96 stars, based on 1 article reviews
    secondary antibody solution - by Bioz Stars, 2026-09
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